6XRW
Chromosomal ParDE TA system from P. aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-25 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 98.401, 121.323, 58.584 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.860 - 1.770 |
R-factor | 0.1799 |
Rwork | 0.177 |
R-free | 0.21920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cze |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
High resolution limit [Å] | 1.770 | 4.880 | 1.800 |
Rmerge | 0.048 | 0.028 | 0.276 |
Rmeas | 0.054 | 0.031 | 0.352 |
Rpim | 0.022 | 0.014 | 0.216 |
Number of reflections | 33497 | 1774 | 1574 |
<I/σ(I)> | 12.8 | ||
Completeness [%] | 99.6 | 99.7 | 95.3 |
Redundancy | 5.1 | 5.3 | 2.2 |
CC(1/2) | 0.999 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1:1 mixture of precipitant 7.5% MPD, 20% PEG 6000, 25 mM (NH4)2SO4, 100 mM Tris pH 7.0 with protein solution 10.5 mg/mL ParDE in 25 mM MES pH 6.0, 150 mM NaCl, 2.5% glycerol |