6X1F
Tubulin-RB3_SLD-TTL in complex with compound 5m
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.480, 157.923, 182.205 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.240 - 2.700 |
R-factor | 0.1915 |
Rwork | 0.190 |
R-free | 0.24080 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 6br1 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.532 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.106 | |
Rpim | 0.045 | |
Number of reflections | 84115 | 4130 |
<I/σ(I)> | 17.5 | 2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.8 | 7 |
CC(1/2) | 0.990 | 0.592 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 6% PEG 4000, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7 |