6WT9
Structure of STING-associated CdnE c-di-GMP synthase from Capnocytophaga granulosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-12-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.998, 48.879, 59.704 |
| Unit cell angles | 90.00, 92.51, 90.00 |
Refinement procedure
| Resolution | 44.850 - 2.300 |
| R-factor | 0.1915 |
| Rwork | 0.186 |
| R-free | 0.23960 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.410 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.850 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.096 | 0.457 |
| Number of reflections | 14608 | 1408 |
| <I/σ(I)> | 17 | |
| Completeness [%] | 99.6 | |
| Redundancy | 10.4 | |
| CC(1/2) | 0.961 | 0.769 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M sodium thiocyanate, 20% PEG-3350 |
