6WQD
The 1.95 A Crystal Structure of the Co-factor Complex of NSP7 and the C-terminal Domain of NSP8 from SARS-CoV-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-23 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.261, 55.103, 84.397 |
Unit cell angles | 90.00, 90.38, 90.00 |
Refinement procedure
Resolution | 41.120 - 1.950 |
R-factor | 0.1887 |
Rwork | 0.187 |
R-free | 0.22870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6wiq |
RMSD bond length | 0.008 |
RMSD bond angle | 0.829 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.850 | 1.950 |
Rmerge | 0.188 | 1.150 |
Number of reflections | 30923 | 1220 |
<I/σ(I)> | 22.5 | 1.1 |
Completeness [%] | 97.3 | 77.9 |
Redundancy | 8.7 | 2.2 |
CC(1/2) | 0.962 | 0.456 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M magnesium chloride, 0.1 M Tris, pH 8.5, 20% w/v PEG8000 |