6WKP
Crystal structure of RNA-binding domain of nucleocapsid phosphoprotein from SARS CoV-2, monoclinic crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-07 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.388, 71.848, 106.961 |
Unit cell angles | 90.00, 92.98, 90.00 |
Refinement procedure
Resolution | 35.610 - 2.670 |
R-factor | 0.2001 |
Rwork | 0.197 |
R-free | 0.24770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vyo |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
High resolution limit [Å] | 2.670 | 7.320 | 2.700 |
Rmerge | 0.091 | 0.043 | 0.597 |
Rmeas | 0.102 | 0.048 | 0.684 |
Rpim | 0.046 | 0.021 | 0.326 |
Number of reflections | 14108 | 746 | 665 |
<I/σ(I)> | 8.2 | ||
Completeness [%] | 97.5 | 98.3 | 90 |
Redundancy | 4.5 | 4.7 | 3.8 |
CC(1/2) | 0.994 | 0.879 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1 M MES, 30% PEG4000 |