6WC3
Crystal structure of the SNARE Sec20 bound to Dsl1 complex subunit Tip20
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-20 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97930 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 133.275, 133.275, 288.470 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.294 - 3.203 |
R-factor | 0.2132 |
Rwork | 0.210 |
R-free | 0.24820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fhn |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHASER (2.5.5) |
Refinement software | PHENIX (1.13.2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.260 | 29.260 | 3.280 |
High resolution limit [Å] | 3.200 | 14.320 | 3.200 |
Rmerge | 0.132 | 0.032 | 1.174 |
Rmeas | 0.135 | 0.033 | 1.200 |
Rpim | 0.026 | 0.007 | 0.244 |
Total number of observations | 666066 | 6567 | 35919 |
Number of reflections | 25597 | 328 | 1638 |
<I/σ(I)> | 27.6 | 90 | 3.2 |
Completeness [%] | 99.0 | 88.6 | 88.4 |
Redundancy | 26 | 20 | 21.9 |
CC(1/2) | 1.000 | 1.000 | 0.878 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.1 M sodium citrate, pH 6.0, 0.725 M ammonium sulfate, 1 mM dithiothreitol, cryoprotected with 30% (v/v) glycerol |