6WAC
FF248-249AA PCNA mutant defective in BIR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2015-04-15 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.000 |
Spacegroup name | H 3 |
Unit cell lengths | 86.881, 86.881, 100.724 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.280 - 2.900 |
R-factor | 0.2383 |
Rwork | 0.237 |
R-free | 0.27010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1plq |
RMSD bond length | 0.003 |
RMSD bond angle | 0.532 |
Data reduction software | TRUNCATE |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | Coot (0.8.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.280 | 3.003 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.079 | 0.837 |
Rpim | 0.057 | 0.604 |
Number of reflections | 12383 | 595 |
<I/σ(I)> | 13.4 | 2 |
Completeness [%] | 99.6 | |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.15 | magnesium chloride hexahydrate, sodium dimethylarsinic acid, PEG1000 |