6WA9
Structure of the Chlamydia pneumoniae CdsV and CdsO protein complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-26 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 156.410, 206.610, 280.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.220 - 4.620 |
R-factor | 0.243 |
Rwork | 0.241 |
R-free | 0.28550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6wa6 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.550 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | PHENIX (1.18.2-3874-000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.270 | 4.700 |
High resolution limit [Å] | 4.620 | 4.620 |
Number of reflections | 50242 | 4970 |
<I/σ(I)> | 8.1 | |
Completeness [%] | 99.3 | |
Redundancy | 7.5 | |
CC(1/2) | 0.998 | 0.570 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294.15 | 100mM Bis-Tris pH 6.0, 4% w/v PEG 3350, 200mM ammonium acetate |