6W1K
Crystal structure of the hydroxyglutarate synthase in complex with 2-oxoadipate from Oryza sativa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 92.300, 98.200, 203.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.790 - 1.850 |
R-factor | 0.1659 |
Rwork | 0.165 |
R-free | 0.19150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lho |
RMSD bond length | 0.011 |
RMSD bond angle | 1.086 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.800 | 1.910 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 156493 | 15364 |
<I/σ(I)> | 11.5 | |
Completeness [%] | 98.9 | |
Redundancy | 7.3 | |
CC(1/2) | 0.998 | 0.517 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 0.01 M Magnesium Chloride, 0.05 M MES pH 5.6, 1.8 M Lithium Sulfate |