6VRS
Single particle reconstruction of glucose isomerase from Streptomyces rubiginosus based on data acquired in the presence of substantial aberrations
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | TFS TALOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2.7 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.7 |
Number of Particle | 114522 |
Software | cisTEM,REFMAC,SerialEM,MOLREP |
Symmetry | D2 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |