6VOQ

Crystal structure of YgbL, a putative aldolase/epimerase/decarboxylase from Klebsiella pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-07-31
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	I 4 2 2
Unit cell lengths	76.436, 76.436, 181.682
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.580 - 2.490
R-factor	0.159
Rwork	0.156
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2opi
RMSD bond length	0.006
RMSD bond angle	0.791
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	BALBES
Refinement software	PHENIX (1.15_3448)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.540
High resolution limit [Å]	2.490	2.500
Rmerge	0.134	0.817
Rpim	0.037	0.233
Number of reflections	9859	490
<I/σ(I)>	18.74	2.59
Completeness [%]	100.0
Redundancy	13.8
CC(1/2)		0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.2M Na Tartrate, 0.1M TRIS pH 8.5, 25% PEG3350