6V3Z
Structure of Salmonella enteritidis Sen1395
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.374, 49.371, 58.639 |
| Unit cell angles | 91.41, 107.55, 93.61 |
Refinement procedure
| Resolution | 49.270 - 1.900 |
| R-factor | 0.20315 |
| Rwork | 0.201 |
| R-free | 0.23616 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TTSA |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.653 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.270 | 1.972 |
| High resolution limit [Å] | 1.900 | 1.904 |
| Rmerge | 0.064 | 0.572 |
| Number of reflections | 26682 | 2484 |
| <I/σ(I)> | 11.25 | |
| Completeness [%] | 89.8 | |
| Redundancy | 2.6 | |
| CC(1/2) | 0.980 | 0.376 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M sodium malonate pH 7.0 and 20% w/v PEG 3,350 |
