6UOL

Y271G DNA polymerase beta substrate complex with a templating cytosine and incoming rGTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-05
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	50.800, 80.400, 55.500
Unit cell angles	90.00, 107.90, 90.00

Refinement procedure

Resolution	25.088 - 1.936
R-factor	0.1927
Rwork	0.186
R-free	0.24960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fms
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.088	25.000	1.980
High resolution limit [Å]	1.936	5.280	1.950
Rmerge	0.042	0.023	0.864
Rmeas	0.047	0.026
Rpim	0.022	0.011	0.677
Number of reflections	42206	1609	1498
<I/σ(I)>	14.3
Completeness [%]	99.3	100	98.2
Redundancy	4	5.5	2.1
CC(1/2)		0.999	0.596

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	19% PEG3350, 0.35 M sodium acetate, 0.05 M Imidazole, pH 8.0.