6UJD
Crystal structure of Cysteine-tRNA ligase from Elizabethkingia sp.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-08-08 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 78.260, 78.260, 208.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.460 - 2.600 |
R-factor | 0.1798 |
Rwork | 0.175 |
R-free | 0.21680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1li5-A as per Morda |
RMSD bond length | 0.004 |
RMSD bond angle | 0.623 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MoRDa |
Refinement software | PHENIX (1.17rc2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.670 |
High resolution limit [Å] | 2.600 | 11.630 | 2.600 |
Rmerge | 0.042 | 0.023 | 0.546 |
Rmeas | 0.045 | 0.025 | 0.583 |
Number of reflections | 20872 | 292 | 1490 |
<I/σ(I)> | 29.87 | 61.84 | 3.69 |
Completeness [%] | 99.9 | 93.6 | 100 |
Redundancy | 7.936 | 5.606 | 8.17 |
CC(1/2) | 1.000 | 0.999 | 0.918 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 290 | Anatrace MCSG-1 screen, condition A3: 10% (w/V) PEG 8000, 200mM sodium chloride, 100mM sodium phosphate dibasic / potassium phosphate monobasic pH 6.2: ElmeA.00133.a.B1.PS385583 at 8.47 mg/ml: cryo: 25% EG in 2 steps: tray 310561 a3: puck kzn9-2. |