6UHQ
Crystal Structure of C148 mGFP-cDNA-3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-12-01 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 106.630, 50.580, 56.690 |
Unit cell angles | 90.00, 110.33, 90.00 |
Refinement procedure
Resolution | 50.040 - 2.850 |
R-factor | 0.1883 |
Rwork | 0.181 |
R-free | 0.27390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5n9o |
RMSD bond length | 0.007 |
RMSD bond angle | 1.748 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 53.159 | 53.159 | 3.000 |
High resolution limit [Å] | 2.850 | 9.010 | 2.850 |
Rmerge | 0.054 | 0.677 | |
Rmeas | 0.212 | 0.066 | 0.802 |
Rpim | 0.112 | 0.037 | 0.421 |
Total number of observations | 21886 | ||
Number of reflections | 6723 | 230 | 976 |
<I/σ(I)> | 5.8 | 11.4 | 2.1 |
Completeness [%] | 99.3 | 98.9 | 99.2 |
Redundancy | 3.3 | 3.1 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 1 microliter C148 mGFP-cDNA-3 (5 mg/mL (protein concentration) in 10 mM Tris Buffer pH 7.4, 137 mM NaCl) + 1 microliter crystallization condition (0.1 M MES pH 6.5, 30% (w/v) PEG 4000) in a sitting drop with a 70 microliter reservoir (0.1 M MES pH 6.5, 30% (w/v) PEG 4000) |