6UHM

Crystal Structure of a Physical Mixture of C148 mGFP and scDNA-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-09
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	58.280, 61.760, 135.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.250 - 2.100
R-factor	0.2139
Rwork	0.211
R-free	0.27010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n9o
RMSD bond length	0.008
RMSD bond angle	1.671
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.660	61.760	2.210
High resolution limit [Å]	2.100	6.640	2.100
Rmerge		0.051	0.503
Rmeas	0.079	0.055	0.534
Rpim	0.026	0.020	0.177
Total number of observations	250177
Number of reflections	29215	1057	4155
<I/σ(I)>	14.5	29	3.7
Completeness [%]	99.8	99.8	99.4
Redundancy	8.6	7.5	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	295	1 microliter of a physical mixture of C148 mGFP and scDNA-1 (5 mg/mL C148 mGFP and equimolar scDNA-1 in 10 mM Tris Buffer pH 7.4, 137 mM NaCl) + 1 microliter crystallization condition (0.1 M sodium acetate, 25% (w/v) PEG 4000, 8% (v/v) isopropanol) in a sitting drop with a 70 microliter reservoir (0.1 M sodium acetate, 25% (w/v) PEG 4000, 8% (v/v) isopropanol)