6UG1
Sequence impact in DNA duplex opening by the Rad4/XPC nucleotide excision repair complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2016-01-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97919 |
Spacegroup name | P 1 |
Unit cell lengths | 53.244, 59.571, 78.240 |
Unit cell angles | 105.51, 97.88, 107.14 |
Refinement procedure
Resolution | 39.412 - 2.833 |
R-factor | 0.2264 |
Rwork | 0.224 |
R-free | 0.27190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 2.113 |
Data reduction software | d*TREK |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 18202 | 18202 |
<I/σ(I)> | 17.35 | |
Completeness [%] | 91.2 | |
Redundancy | 2.9 | |
CC(1/2) | 0.744 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 50 mM BTP-HCl, 150 mM sodium chloride, 12% isopropanol, 100 mM calcium chloride |