6TWS
Crystal structure of the haemagglutinin mutant (Gln226Leu, Gly228Ser) from an H10N7 seal influenza virus isolated in Germany with 2mM EDTA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.148, 215.596, 72.870 |
| Unit cell angles | 90.00, 100.46, 90.00 |
Refinement procedure
| Resolution | 61.410 - 2.000 |
| R-factor | 0.2103 |
| Rwork | 0.209 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d00 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.686 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.410 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 131081 | 6478 |
| <I/σ(I)> | 12.7 | 3.3 |
| Completeness [%] | 98.8 | |
| Redundancy | 7 | |
| CC(1/2) | 0.998 | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 6% PEG3350, 0.1 M MES pH6.5 |
