6TV1
ATPase domain D3 of the EssC coupling protein from S. aureus USA300
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 91.030, 91.030, 124.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.751 - 1.700 |
R-factor | 0.1538 |
Rwork | 0.153 |
R-free | 0.17240 |
Structure solution method | SAD |
Data scaling software | XDS |
Phasing software | PHENIX (1.15.2_3472) |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.751 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.045 | 1.559 |
Rmeas | 0.048 | 1.743 |
Rpim | 0.016 | 0.533 |
Number of reflections | 57937 | 4176 |
<I/σ(I)> | 25.71 | 1.19 |
Completeness [%] | 99.7 | 98.72 |
Redundancy | 8.7 | 8.7 |
CC(1/2) | 1.000 | 0.539 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 1,4-1,8 M ammonium citrate |