6TF7
Human galectin-3c in complex with a galactose derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-27 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.03841 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.140, 58.160, 62.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.080 - 1.400 |
| R-factor | 0.1374 |
| Rwork | 0.135 |
| R-free | 0.18720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zsl |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.184 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC (5) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.080 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.048 | 0.519 |
| Number of reflections | 26601 | 1923 |
| <I/σ(I)> | 24 | |
| Completeness [%] | 99.6 | |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 20 MG/ML GALECTIN-3C, 100MM NACL, 30 % W/V PEG 4000, 0.4 M NASCN, 10 MM BETA-MERCAPTOETHANOL, 10 MM PHOSHATE, 100 MM MGCL2, 100 MM TRIS/HCL PH 7.5, 4.5 MM LIGAND |
