6T3J
Dual Epitope Targeting by Anti-DR5 Antibodies
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.00001 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 121.604, 90.926, 138.379 |
Unit cell angles | 90.00, 103.79, 90.00 |
Refinement procedure
Resolution | 134.410 - 3.050 |
R-factor | 0.2508 |
Rwork | 0.251 |
R-free | 0.28190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.341 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 134.410 | 3.300 |
High resolution limit [Å] | 3.050 | 3.050 |
Rmerge | 0.088 | 0.820 |
Rmeas | 0.107 | 0.024 |
Number of reflections | 55388 | 11644 |
<I/σ(I)> | 10.69 | 1.77 |
Completeness [%] | 98.5 | 99 |
Redundancy | 3 | 3.1 |
CC(1/2) | 0.997 | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 281 | 10% (w/v) PEG 4000 200 mM ammonium sulphate 100 mM Sodium acetate pH 4.6 |