6SY9
Structure of the Legionella pneumophila response regulator LqsR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-17 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.100, 67.840, 116.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.031 - 2.100 |
R-factor | 0.2035 |
Rwork | 0.202 |
R-free | 0.22820 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | HKL2Map |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.031 | 46.031 | 2.150 |
High resolution limit [Å] | 2.100 | 9.390 | 2.100 |
Rmerge | 0.089 | 0.020 | 1.950 |
Rmeas | 0.093 | 0.021 | 2.027 |
Total number of observations | 311660 | ||
Number of reflections | 23899 | 318 | 1732 |
<I/σ(I)> | 20.98 | 83.16 | 1.67 |
Completeness [%] | 100.0 | 99.1 | 100 |
Redundancy | 13.041 | 10.151 | 13.577 |
CC(1/2) | 1.000 | 1.000 | 0.639 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Sodium formate, 0.1M Bis-Tris propane, 18% (w/v) PEG 3350 |