6SWT
Affimer9 co-crystalised with the CH domains of alpha actinin 2.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9159 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.192, 48.519, 146.993 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.500 | 1.230 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.073 | 0.802 |
Rmeas | 0.079 | 0.948 |
Rpim | 0.029 | 0.493 |
Number of reflections | 103011 | 7078 |
<I/σ(I)> | 10.4 | 1.6 |
Completeness [%] | 98.9 | 93.3 |
Redundancy | 5.9 | 3.4 |
CC(1/2) | 0.997 | 0.622 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350, 0.2M ammonium tartrate |