6SEK
Crystal Structure of Ancestral Flavin-containing monooxygenase (FMO) 5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 96.782, 100.069, 143.152 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.000 - 2.700 |
R-factor | 0.22586 |
Rwork | 0.222 |
R-free | 0.29453 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.900 | 2.820 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.110 | |
Number of reflections | 38832 | 4655 |
<I/σ(I)> | 9.9 | 0.8 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.6 | 6.9 |
CC(1/2) | 0.999 | 0.421 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | PEG 4000 HEPES |