6SB5
CryoEM structure of murine perforin-2 ectodomain in a pore form
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK III |
EM Data Collection
Microscope | FEI TALOS ARCTICA |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 5 |
Software | RELION,EPU,Gctf |
Symmetry | C16 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |