6SA4
SALSA / DMBT1 / GP340 SRCR domain 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2018-01-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0718 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.770, 45.190, 69.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.520 - 1.770 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a2e |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.809 |
| Data reduction software | xia2 (3d) |
| Data scaling software | xia2 (3d) |
| Phasing software | MOLREP (CCP4Interface 7.0.056) |
| Refinement software | PHENIX (dev_3126) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.520 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.170 | 1.364 |
| Number of reflections | 11730 | 1146 |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 0.2 M MgCl2 hexahydrate, 10 5 (w/v) PEG8000, 0.1 M Tris, pH 7.0 |
