6S6D
Crystal structure of RagA-Q66L-GTP/RagC-S75N-GDP GTPase heterodimer complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.504, 81.439, 246.033 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.290 - 2.500 |
R-factor | 0.2127 |
Rwork | 0.209 |
R-free | 0.28660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.704 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.290 | 49.290 | 2.650 |
High resolution limit [Å] | 2.500 | 7.420 | 2.500 |
Rmerge | 0.070 | 0.033 | 1.047 |
Rmeas | 0.077 | 0.036 | 1.136 |
Total number of observations | 331756 | ||
Number of reflections | 50589 | 2092 | 8018 |
<I/σ(I)> | 16.69 | 45.88 | 1.94 |
Completeness [%] | 99.8 | 98.7 | 99 |
Redundancy | 6.558 | 5.576 | 6.671 |
CC(1/2) | 0.999 | 0.998 | 0.654 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM MOPSO, Bis-Tris pH 6.5, 40mM polyamines ( mixture of spermine tetrahydrochloride, spermidine trihydrochloride, 1,4. diaminobutane dihydrochloride, 0.1 M D/L-ornithine monohydrochloride), 10% (w/v) PEG 8000. 20% (v/v) 1,5 - Pentanediol and 24 % (v/v) glycerol. |