6RZY

Plasmodium falciparum PFA0660w Hsp40 co-chaperone J-domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0389
Spacegroup name	P 1 21 1
Unit cell lengths	25.828, 54.925, 51.441
Unit cell angles	90.00, 98.23, 90.00

Refinement procedure

Resolution	37.338 - 1.379
R-factor	0.1665
Rwork	0.165
R-free	0.20270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rwu
RMSD bond length	0.008
RMSD bond angle	1.027
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.925	1.403
High resolution limit [Å]	1.379	1.379
Rmerge	0.072	0.639
Rmeas	0.086	0.772
Rpim	0.046	0.427
Number of reflections	28692	1444
<I/σ(I)>	9.5	2.1
Completeness [%]	97.7	99.1
Redundancy	3.3	3.1
CC(1/2)	0.997	0.495

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.12 M mix of ethylene glycols 0.1 M Tris (base)/Bicine 30% w/v PEG MME550 30% w/v PEG 20,000