6RRQ
Crystal structure of tyrosinase PvdP from Pseudomonas aeruginosa bound to copper
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-22 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.722, 114.215, 100.906 |
Unit cell angles | 90.00, 94.82, 90.00 |
Refinement procedure
Resolution | 49.660 - 2.700 |
R-factor | 0.1897 |
Rwork | 0.186 |
R-free | 0.25960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rrr |
RMSD bond length | 0.011 |
RMSD bond angle | 1.467 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.2) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.660 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.087 | 0.552 |
Rmeas | 0.102 | 0.650 |
Rpim | 0.052 | 0.339 |
Number of reflections | 31354 | 4186 |
<I/σ(I)> | 11.1 | |
Completeness [%] | 99.3 | 99.1 |
Redundancy | 3.7 | 3.6 |
CC(1/2) | 0.996 | 0.795 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 20% PEG 3350, 0.1M Bis-Tris Propane |