6RMC
Crystal structure of the DEAH-box ATPase Prp2 in complex with Spp2 and ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-08-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.730, 113.690, 191.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.896 - 2.600 |
R-factor | 0.2235 |
Rwork | 0.222 |
R-free | 0.25040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4YB2 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.539 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.710 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.069 | 0.930 |
Rmeas | 0.077 | |
Total number of observations | 256605 | |
Number of reflections | 50939 | 6069 |
<I/σ(I)> | 17.33 | 1.83 |
Completeness [%] | 99.6 | 99.7 |
Redundancy | 5.04 | |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 100 mM Bicine/Trizma pH 7.5, 11% (w/v) PEG8000, 20% (w/v) ethylene glycol, 20 mM of a monosaccharide mixture (D-glucose, D-mannose, D-galactose, L-fucose, D-xylose and N-acetyl-D-glucosamine) |