6R44
Structure of the SBP FpvC in complex with Ni2+ ion from P.aeruginosa from P21 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.920, 41.380, 104.620 |
Unit cell angles | 90.00, 95.85, 90.00 |
Refinement procedure
Resolution | 43.190 - 1.990 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6r3z |
RMSD bond length | 0.010 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.190 | 2.050 |
High resolution limit [Å] | 1.990 | 1.990 |
Number of reflections | 38967 | 8580 |
<I/σ(I)> | 6.13 | |
Completeness [%] | 99.1 | |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 16% PEG 6K, 0.1 M MES |