6QZJ
Crystal structure of human Aquaporin 7 at 2.2 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | I 4 |
Unit cell lengths | 86.761, 86.761, 83.544 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.772 - 2.200 |
R-factor | 0.2105 |
Rwork | 0.209 |
R-free | 0.24320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6f7h |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.350 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.027 | 0.673 |
Rmeas | 0.032 | 0.797 |
Rpim | 0.016 | 0.418 |
Number of reflections | 15584 | 1364 |
<I/σ(I)> | 17.2 | |
Completeness [%] | 98.8 | 99.6 |
Redundancy | 3.3 | 3.4 |
CC(1/2) | 1.000 | 0.788 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 85 mM Tris pH 8.0, 37 % v/v PEG 200 and 0.80 % v/v OG |