6QJ0
Crystal structure of the C. thermophilum condensin Smc2 ATPase head (crystal form II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 65 |
Unit cell lengths | 93.745, 93.745, 117.971 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 66.879 - 2.000 |
R-factor | 0.1907 |
Rwork | 0.189 |
R-free | 0.22150 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.855 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 81.186 | 81.186 | 2.110 |
High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
Rmerge | 0.064 | 1.649 | |
Rmeas | 0.103 | 0.067 | 1.733 |
Rpim | 0.032 | 0.021 | 0.533 |
Total number of observations | 409294 | ||
Number of reflections | 39710 | 1299 | 5768 |
<I/σ(I)> | 12.3 | 6.8 | 0.5 |
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 10.3 | 10.1 | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10-17 % (w/v) PEG 8,000, 0.1 M Na cacodylate pH 6.0 |