6QFK
EngBF DARPin Fusion 4b G10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 65 |
Unit cell lengths | 192.893, 192.893, 122.922 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.250 - 2.000 |
R-factor | 0.155 |
Rwork | 0.154 |
R-free | 0.17600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zxq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.330 | 2.072 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.254 | 2.903 |
Number of reflections | 174930 | |
<I/σ(I)> | 13.46 | |
Completeness [%] | 100.0 | |
Redundancy | 20.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.6 | 277.15 | MPD, PEG 20,000, MES, sodium chloride, manganese chloride |