6Q8M
GH10 endo-xylanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-09-23 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97965 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.342, 74.073, 213.902 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.090 - 1.420 |
R-factor | 0.1489 |
Rwork | 0.148 |
R-free | 0.17049 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u7b |
RMSD bond length | 0.015 |
RMSD bond angle | 1.934 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.090 | 1.440 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.071 | |
Rpim | 0.032 | 1.090 |
Number of reflections | 142509 | |
<I/σ(I)> | 15.7 | 2.3 |
Completeness [%] | 99.7 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | sodium/potassium phosphate (pH 6.9) |