6Q0I
Inferred intermediate I-7 (I-7-6) of the human antibody lineage 652 in complex with influenza hemagglutinin head domain of A/Beijing/262/95(H1N1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2018-06-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.950, 70.410, 203.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.881 - 2.702 |
R-factor | 0.2156 |
Rwork | 0.213 |
R-free | 0.25540 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.881 | 2.799 |
High resolution limit [Å] | 2.702 | 2.702 |
Rmerge | 0.072 | 1.038 |
Number of reflections | 21279 | 18093 |
<I/σ(I)> | 24.15 | |
Completeness [%] | 99.8 | 99.23 |
Redundancy | 8.7 | |
CC(1/2) | 0.999 | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291.15 | 100mM lithium sulfate, 30% (w/v) PEG 6000, 100mM CHES |