6Q0E
Inferred precursor (UCA) of the human antibody lineage 652 in complex with influenza hemagglutinin head domain of A/Beijing/262/95(H1N1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 291.15 |
Detector technology | CCD |
Collection date | 2017-05-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.030, 69.780, 200.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.292 - 2.150 |
R-factor | 0.2312 |
Rwork | 0.230 |
R-free | 0.24850 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.292 | 2.227 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.141 | |
Number of reflections | 40694 | 4005 |
<I/σ(I)> | 13.21 | |
Completeness [%] | 99.5 | 99.78 |
Redundancy | 7.05 | |
CC(1/2) | 0.997 | 0.801 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.15 | 100 mM sodium chloride, 30% (v/v) PEG 400, 100 mM MES |