6PID
Crystal structure of Marinobacter subterrani acetylpolyamine amidohydrolase (msAPAH) complexed with 8-amino-N-hydroxyoctanamide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-10 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.330, 119.060, 66.220 |
Unit cell angles | 90.00, 109.24, 90.00 |
Refinement procedure
Resolution | 59.530 - 1.546 |
R-factor | 0.2447 |
Rwork | 0.244 |
R-free | 0.28050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB: 4ZUM |
RMSD bond length | 0.006 |
RMSD bond angle | 0.850 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.530 | 1.600 |
High resolution limit [Å] | 1.540 | 1.550 |
Rpim | 0.172 | 0.742 |
Number of reflections | 95821 | 7699 |
<I/σ(I)> | 5.6 | |
Completeness [%] | 88.7 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10 mg/ml msAPAH Protein, 0.2 M magnesium acetate tetrahydrate, 20% w/v PEG 3350, 1:1 ratio protein to precipitant |