6PFP
Crystal Structure of Amino Acids 1473-1536 of Human Beta Cardiac Myosin Fused to Gp7 and Eb1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-08 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 |
Unit cell lengths | 37.107, 59.304, 86.548 |
Unit cell angles | 81.79, 86.42, 89.97 |
Refinement procedure
Resolution | 37.177 - 2.200 |
R-factor | 0.1962 |
Rwork | 0.194 |
R-free | 0.24540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | INOH |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
Rmerge | 0.092 | 0.062 | 0.306 |
Rmeas | 0.106 | 0.073 | 0.353 |
Rpim | 0.050 | 0.038 | 0.172 |
Total number of observations | 128524 | ||
Number of reflections | 35454 | 1807 | 1499 |
<I/σ(I)> | 7.1 | ||
Completeness [%] | 95.5 | 97.7 | 83.8 |
Redundancy | 3.6 | 4 | 2.7 |
CC(1/2) | 0.988 | 0.940 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | Crystals grew from 1:1 mixture of 18 mg/ml protein solution with 37% (w/v) 2-methyl-2,4-pentanediol (MPD), 80 mM magnesium sulfate, 100 mM sodium acetate. Protein solution contained 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) pH 7.6, and 0.2 mM tris(2-carboxyethyl)phosphine (TCEP). |