6PD3
Crystal Structure of a H5N1 influenza virus hemagglutinin at pH 5.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 109.383, 109.383, 421.035 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.790 - 2.300 |
| R-factor | 0.20014 |
| Rwork | 0.199 |
| R-free | 0.22887 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fk0 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.544 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.087 | 0.785 |
| Number of reflections | 43303 | 6554 |
| <I/σ(I)> | 11.78 | 2.09 |
| Completeness [%] | 99.1 | 94.7 |
| Redundancy | 6.02 | 5.76 |
| CC(1/2) | 0.998 | 0.884 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 100 mM cacodylate, pH 5.5, 200 mM sodium chloride, 2 M ammonium sulfate, 10% glycerol |
