6OX3
SETD3 in Complex with an Actin Peptide with His73 Replaced with Lysine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.233, 175.748, 66.471 |
Unit cell angles | 90.00, 92.39, 90.00 |
Refinement procedure
Resolution | 36.644 - 1.785 |
R-factor | 0.2085 |
Rwork | 0.208 |
R-free | 0.24650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mbj |
RMSD bond length | 0.004 |
RMSD bond angle | 0.690 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.650 | 1.850 |
High resolution limit [Å] | 1.785 | 1.785 |
Rmerge | 0.253 | 0.983 |
Rpim | 0.089 | 0.654 |
Number of reflections | 127675 | 12113 |
<I/σ(I)> | 9.2 | 1.7 |
Completeness [%] | 98.4 | 93.6 |
Redundancy | 7.1 | 2.5 |
CC(1/2) | 0.419 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 292 | 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000 |