6OX1
SETD3 in Complex with an Actin Peptide with Target Histidine Partially Methylated
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.308, 175.865, 66.967 |
Unit cell angles | 90.00, 92.38, 90.00 |
Refinement procedure
Resolution | 31.268 - 1.949 |
R-factor | 0.1708 |
Rwork | 0.170 |
R-free | 0.20110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mbj |
RMSD bond length | 0.006 |
RMSD bond angle | 0.743 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.15.2_3472)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.270 | 2.020 |
High resolution limit [Å] | 1.949 | 1.949 |
Rmerge | 0.146 | 0.822 |
Rpim | 0.083 | 0.499 |
Number of reflections | 99091 | 9993 |
<I/σ(I)> | 11.7 | 2.9 |
Completeness [%] | 98.3 | 99.4 |
Redundancy | 3.7 | 3.3 |
CC(1/2) | 0.461 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 292 | 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000 |