6OWW
Crystal structure of a Human Cardiac Calsequestrin Filament Complexed with Ytterbium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.3857 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.832, 86.015, 214.340 |
Unit cell angles | 90.00, 89.91, 90.00 |
Refinement procedure
Resolution | 214.340 - 3.840 |
R-factor | 0.2922 |
Rwork | 0.290 |
R-free | 0.34010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6owv |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 214.340 | 214.670 | 3.910 |
High resolution limit [Å] | 3.840 | 10.420 | 3.840 |
Rmeas | 0.548 | 0.126 | 3.402 |
Rpim | 0.162 | 0.038 | 0.990 |
Total number of observations | 16616 | 17332 | |
Number of reflections | 29912 | 1575 | 1499 |
<I/σ(I)> | 4.5 | 19.2 | 0.8 |
Completeness [%] | 98.9 | 99.1 | 97.8 |
Redundancy | 11.2 | 10.5 | 11.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 4000, lithium sulfate |