6OV2
Crystal structure of human claudin-9 in complex with Clostridium perfringens entertoxin C-terminal domain in closed form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-05 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.11583 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.940, 111.570, 121.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.096 - 3.200 |
R-factor | 0.2821 |
Rwork | 0.281 |
R-free | 0.29140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Homology model and PDB entry 3AM2 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.769 |
Data reduction software | XDS (0.82) |
Data scaling software | XSCALE (January 26, 2018) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 3.240 |
High resolution limit [Å] | 3.160 | 3.160 |
Rmeas | 0.041 | 6.589 |
Number of reflections | 30828 | 2276 |
<I/σ(I)> | 5.8 | 0.18 |
Completeness [%] | 96.4 | 96.9 |
Redundancy | 9.6 | 6 |
CC(1/2) | 0.990 | 0.123 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 150 mM sodium acetate, sodium cacodylate, Bis-Tris propane, pH 4.5, 25% PEG1500 |