6OPK
Phosphorylated ERK2 with Vertex-11e
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.986, 78.139, 152.601 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.540 |
| R-factor | 0.19562 |
| Rwork | 0.193 |
| R-free | 0.24081 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2erk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.657 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.098 | 0.930 |
| Rmeas | 1.054 | |
| Rpim | 0.490 | |
| Number of reflections | 19482 | 1403 |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 16.5% PEG3350,100 mM Tris-HCl pH 8.5 |
