6NU8

Structure of sucrose-6-phosphate hydrolase from Lactobacillus gasseri in complex with fructose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.4587
Spacegroup name	P 4 21 2
Unit cell lengths	103.860, 103.860, 102.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.890 - 1.800
R-factor	0.13596
Rwork	0.134
R-free	0.16532
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.816
Data reduction software	TRUNCATE
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.890	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.092	0.272
Rmeas	0.096	0.345
Rpim	0.029	0.209
Number of reflections	51784	2668
<I/σ(I)>	17	2.7
Completeness [%]	99.1	88.2
Redundancy	18	4.3
CC(1/2)	0.999	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1 M buffer MMT pH 9.0; 25% (m/v) PEG 1500