6NHK

Mortalin nucleotide binding domain in the ADP-bound state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-02
Detector	NOIR-1
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	177.263, 60.102, 91.699
Unit cell angles	90.00, 97.56, 90.00

Refinement procedure

Resolution	56.868 - 2.777
R-factor	0.2588
Rwork	0.256
R-free	0.29580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3atu
RMSD bond length	0.003
RMSD bond angle	0.607
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.870	2.877
High resolution limit [Å]	2.777	2.777
Number of reflections	24320	2310
<I/σ(I)>	13.61	1.1
Completeness [%]	99.1	94
Redundancy	3.7	3.4
CC(1/2)	0.998	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.17 M Ammonium Acetate, 0.085 M Sodium Citrate:HCl pH 5.6, 25.5% (w/v) PEG 4000, 15% (v/v) Glycerol