6MOT
Bacteroides intestinalis feruloyl esterase, Bacint_01033
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-07 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.979 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 99.959, 99.959, 164.204 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 86.567 - 1.710 |
R-factor | 0.1713 |
Rwork | 0.171 |
R-free | 0.19120 |
Structure solution method | SAD |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | SOLVE |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.567 | 1.730 |
High resolution limit [Å] | 1.710 | 1.710 |
Number of reflections | 99242 | |
<I/σ(I)> | 19.3 | |
Completeness [%] | 100.0 | |
Redundancy | 40.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 1.5M Lithium sulfate, 0.1 M sodium acetate (Hampton SaltRx B10) |