6MOF
Monomeric DARPin G2 complex with EpoR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 108.834, 108.834, 96.264 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.053 - 2.894 |
R-factor | 0.2279 |
Rwork | 0.224 |
R-free | 0.26570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ern predicted model of G2 |
RMSD bond length | 0.001 |
RMSD bond angle | 0.441 |
Data reduction software | XDS (Jun 1, 2017) |
Data scaling software | XDS (Jun 1, 2017) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.053 | 2.998 |
High resolution limit [Å] | 2.894 | 2.894 |
Rmerge | 0.309 | 1.172 |
Rpim | 0.089 | 0.353 |
Number of reflections | 13260 | 1269 |
<I/σ(I)> | 18.83 | 1.18 |
Completeness [%] | 98.7 | 96.87 |
Redundancy | 12.2 | 11.7 |
CC(1/2) | 0.999 | 0.375 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 295 | 0.2 M Magnesium chloride, 0.1 M Tris pH 7.0, 10% PEG 8000, 25% ethylene glycol as cryoprotectant |