6ML2
ZBTB24 Zinc Fingers 4-8 with 19+1mer DNA Oligonucleotide (Sequence 1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.065, 68.619, 49.477 |
Unit cell angles | 90.00, 91.90, 90.00 |
Refinement procedure
Resolution | 38.232 - 1.874 |
R-factor | 0.2523 |
Rwork | 0.251 |
R-free | 0.27810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5V3G (zinc fingers) + 18-mer B-DNA model |
RMSD bond length | 0.005 |
RMSD bond angle | 0.644 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.120 | 1.940 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.056 | 0.564 |
Rpim | 0.024 | 0.264 |
Number of reflections | 36754 | 699 |
<I/σ(I)> | 22.6 | 2.1 |
Completeness [%] | 77.7 | 27.6 |
Redundancy | 5.6 | 3.9 |
CC(1/2) | 0.864 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 25% PEG3350, 0.1 M Bis-Tris, pH 6.5 |